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KMID : 0380620140460030384
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Korean Journal of Food Science and Technology
2014 Volume.46 No. 3 p.384 ~ p.389
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Protective Effect of Radiation-induced New Blackberry Mutant γ-B201 on H2O2-induced Oxidative Damage in HepG2 Cells
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Cho Byoung-Ok
Lee Chang-Wook
So Yang-kang
Jin Chang-Hyun
Yook Hong-Sun
Byun Myung-Woo
Jeong Yong-Wook
Park Jong-Chun
Jeong Il-Yun
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Abstract
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The objective of the present study was to investigate the chemical composition of anthocyanin-enriched extract of radiation-induced blackberry (Rubus fruticosus L.) mutant (γ-B201) as well as the protective effect of γ-B201 against oxidative stress in vitro. The cytotoxicity, reactive oxygen species (ROS) scavenging capacity, and DNA damage were assessed by WST-1 assay, flow cytometry, and comet assay, respectively. Lactate dehydrogenase, superoxide dismutase, and catalase activities were determined by using a commercial kit. The in vitro results showed that γ-B201 increased the cell viability, reduction of lactate dehydrogenase release, and intracellular ROS scavenging capacity in hydrogen peroxide (H2O2 )-treated HepG2 cells. Furthermore, treatment with γ-B201 attenuated DNA damage in H2O2-treated HepG2 cells and treatment with γ-B201 restored the activity of superoxide dismutase and catalase in H2O2-treated HepG2 cells. In conclusion, the present study suggests that γ-B201 blackberry extract can exert a significant cytoprotective effect against H2O2-induced cell damage.
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KEYWORD
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blackberry, ROS, cell viability, DNA damage, antioxidant enzyme
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